Performing a risk assessment laboratory biosafety manual, 3rd edition, 2004 link to world health organization website guidelines for safe work practices in human and animal medical diagnostic laboratories link to cdc website. Biosafety in microbiological and biomedical laboratories bmbl, section vi, 5th edition, february 2007. Centres for disease control and prevention, national institute of health, u. Biosafety in microbiological and biomedical laboratories uab. General and specific biosafety guidelines for handling sarscov2 specimens are provided below. These guidelines for biosafety laboratory competency outline the essential skills, knowledge, and abilities required for working with biologic agents at the three highest biosafety levels bsls levels 2. Department of health and human cch laboratory safety manual. June 2010 page 1 of 185 institutional biosafety manual the purpose of this manual is to define the biological safety policies and procedures pertaining to research operations at boston university and boston medical center. A report for trust working towards equitable north south research partnerships. June 2010 page 3 of 185 appendix a ibc application forms 89 appendix b importation and exportation of etiologic agents 90 appendix c laboratory ventilation and containment for biosafety 93 appendix d autoclave quality assurance program 100 appendix e biosafety level 2 bsl2 requirements 103. There are no specific guidelines for labs not working with gmos, and there is no. The canadian biosafety handbook cbh, 2 nd edition, 2016 is intended as a companion document to the canadian biosafety standard cbs, 2 nd edition, 2015.
The biosafety policy and the biosafety program at ohio university ou were established to prevent infection, promote safety in research studies, ensure safe handling of biological agents, provide for safe disposal of infectious laboratory wastes. The biological risk assessment may be quantitative, using mathematical models oie, 2010b. Virtual manual can be used to easily generate a general laboratory safety manual. Cdcaphl laboratory biosafety competencies for the bsl2.
Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Institutional biological safety manual 2010 table of contents. During the past 10 years, laboratory capacity in the region has progressively improved due to a heightened awareness of the need for laboratory capacity, and the strengthening and expansion of the gpln and gisrs. Laboratory manual for the examirriltion of human semcn and sperm cervical mucus interaction. Standards and guidelines for physical containment facilities.
The biosafety manual must be available and accessible. Biosafety for clinical laboratories 2010 powerpoint presentation to viewprint. Provides protection for the worker and the laboratory environment but not to product being utilized in the cabinet. The goals of the biological safety program, referenced in this manual as the biosafety program, are to protect laboratory workers, the public, and the environment from potentially hazardous biological agents. Each facility will have their own inspection reports. Biological safety regulation version 1 current version. Biosafety level refers to the stringency of biocontainment precautions deemed necessary by the centers for disease control and prevention cdc for laboratory work with infectious materials. The development of this third edition of the laboratory biosafety manual has been made possible through the contributions of the following, whose expertise is gratefully acknowledged. Biosafety manual may 2010 page 2 class i biosafety cabinet an enclosure with an inward airflow through the front opening. This includes a fire extinguisher, fire blanket, eyewash station, safety shower and gas cutoff. Chapter title page table of contents 2 emergency phone numbers and resources 34 1 biological safety program 510 2 approval of research projects 1117 3 regulations and guidelines 1823 4 biosafety principles 2431 5 laboratory biosafety practices 3249 6 laboratory training 5053 7 decontamination and sterilization 5458 8 biohazardous spill response 5966. Guidelines for biosafety and laboratory competency. Information available from animal studies and reports of laboratoryacquired. No or low individual and community risk a microorganism that is unlikely to cause.
Laboratory biosafety manual states that no biosafety cabinet or other facility or procedure alone guarantees safety unless the users operate safe techniques based on informed understanding. During this course you will be working with microorganisms that are classifed as biosafety level i and biosafety level ii organisms. Definitions risk groups, biosafety level risk assessment. The biosafety manual is designed in accordance with nih guidelines for research involving recombinant or synthetic nucleic acid molecules and the biosafety in microbiological and biomedical laboratories bmbl, 5th edition to assure laboratory personnel that with proper precautions, equipment, and facilities most biohazardous materials can be handled without undue risk to. Report on the status of edpln bsl3 in select countries in the african region. Footnote 5 it is a guidance document for canadian facilities where human and terrestrial animal pathogens are handled, that updates the guidelines originally published as part ii of the. The ibc advocates the use of biosafety precautions that effectively reduce or. Laboratory specific biosafety manual checklist each laboratory is required to have a labspecific biosafety manual and cannot be borrowed or copied from another lab. Guidance on regulations for the transport of infectious substances previous versions publication date.
Biosafety records records that are retained as required by regulatory and institutional policies for. Biosafety for clinical laboratories 2010 powerpoint presentation to viewprint pdf documentation you will need adobe reader get weekly restaurant inspections and more by email. Biosafety manual laboratory manual that gives practical guidance on overall laboratory operation to provide a safe environment for the use of biological materialsrecombinant dna. The african region experiences around 100 public health events annually, of which 80% are caused by infectious diseases. Biological laboratory safety training risk group classification nih guidelines for research involving recombinant dna molecules, 2011 who laboratory biosafety manual, 3rd edition risk group 1 agents that are not associated with disease in healthy adult humans. The cbsg was developed to update and harmonize three existing canadian biosafety standards and guidelines for the design, construction, and operation of facilities in which. Mssm biosafety manual 2nd edition 42010 table of contents.
The biosafety manual provides detailed information on the specific practices, procedures, physical and engineering controls and protective equipment to be followed. Biological safety will report adverse events to federal or state authorities as required. Mdh laboratories administration laboratory safety manual. Laboratory biosafety manual world health organization. The biosafety manual is designed in accordance with nih guidelines for research involving recombinant or synthetic nucleic acid molecules and the biosafety in microbiological and biomedical laboratories bmbl, 5th edition to assure laboratory personnel that with proper precautions.
Biosafety manual the princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. Class ii biosafety cabinet an enclosure with an inward airflow through the front opening. A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for working with biological agents. Chinese, english, french, portuguese, russian, spanish recommendations for investigating contacts of persons with infectious tuberculosis in low and middleincome countries. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical, research and epidemiological purposes. Guidance on regulations for the transport of infectious substances. Sample laboratory biosafety manual for microbiological. Reports of significant problems or violations are reported to oba within 30 days of the incident. For this new edition, the manual has been extensively revised and expanded. Biosafety in the laboratory is a concise set of practical guidelines for handling and disposing of biohazardous material. The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with bsl2. Who laboratory manual for the examination and processing of human semen 5th ed. Safe use of biological safety cabinets handout 782kb. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels.
Laboratory biosafety and biosecurity risk assessment. Emmett barkley, howard hughes medical institute, chevy chase, md, usa dr murray l. The consensus of top experts in laboratory safety, this volume provides the information needed for immediate improvement of safety practices. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. The virtual manual vm can be used to create the core of your biosafety manual.
Biosafety level bsl and animal biosafety level absl laboratories for research and diagnostic work using developed laboratory inspection checklists. Although only a portion of these public health events are. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. An adequate biosafety manual will contain general biosafety information, as well as information specific to the biological agents and procedures used in the lab. Biosafety and biosecurity center for global health. Who 2010 responsible life sciences research for global health security. In 2006 the who issued a biorisk management document entitled laboratory biosecurity guidance as a complement of laboratory biosafety manual 3 rd edition who, 2006. Its primary focus should be to provide pertinent information to help execute the operations of the lab in a safe and professional manner. The third edition of the who laboratory biosafety manual is a helpful reference.
The manual now covers risk assessment and safe use of recombinant dna technology. Laboratoryspecific biosafety manual checklist each laboratory is required to have a labspecific biosafety manual and cannot be borrowed or copied from another lab. Tuberculosis laboratory biosafety manual languages. As a result of the new laboratory inspection program, ehs was able to increase the number of laboratory inspections it conducted from previous years. Adapted from biosafety manual, lawrence berkeley national laboratory, 2010. Using toxichazardous substancesinfectious agentsrecombinant dna 27 c. The primary purpose of the biosafety manual is to provide an appropriate training and reference tool relative to biosafety for laboratory staff, as discussed in the ehs sop, biosafety training.
The development of this fourth edition of the laboratory biosafety manual has been. In addition, you should be aware of all exits from the rooom. Laboratory biosafety level criteria the essential elements of the four biosafety levels for activities involving infectious microorganisms and laboratory animals are summarized in table 1 of this section and table 1. The biosafety manual is not a replacement or substitute for a general laboratory safety manual, which covers other occupational hazards in the laboratory. The nih office of biotechnology activities oba must. These and other terms are defined in the glossary of this terrestrial manual. In 2010, the government of pakistans planning commission issued a report. Sulkin, harding and byers reported that only a small number of the lai. Review of laboratory biosafety manuals and standard operating procedures sops for compliance with guidelines for bsl and absl procedures. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials. Department of health and human services centers for disease control and prevention morbidity and mortality weekly report guidelines for biosafety. In 2007 a code of conduct for biosecurity has been proposed by the royal netherlands academy of arts and sciences to the scientific community. This research laboratory manual serves as a resource and reference to assist in the. Text was taken from osha bloodborne pathogen standard 29 cfr 1910.
Canadian biosafety handbook, second edition canada. Biosafety manual office of environmental health and safety. It discusses high and lowrisk biological agents including the highestrisk materials handled in labs today, presents the seven. Canadian biosafety standard cbs second edition canada. Mssm biosafety manual 2nd edition 4 2010 table of contents.
For additional information on handling sarscov2 specimens, refer to the laboratory biosafety frequently asked questions. Report on the status of edpln bsl3 in select countries in. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and cetification of laboratories. Biosafety resources mississippi state department of health. Biosafety manual california institute of technology. Who biosafety manual updates 12 october 2015 who strategic framework for action 20122016 who extended biosafety advisory group bag, 2010 regulatory and oversight mechanisms many countries remain without laboratory design and operating parameters often confusing. However, the ebola virus disease outbreak 20142015 highlighted significant gaps in regional laboratory capacity. Biosafety in microbiological and biochemical laboratories, 4th ed. Manual, the us biosafety in microbiological and biomedical laboratories, and the international consensus document cen workshop agreement cwa 15793.
Mycobacteriology laboratory manual v list of abbreviations and acronyms afb acid fast bacilli ast antimycobacterial susceptibility testing. A laboratoryspecific biosafety manual must be prepared and adopted as policy. In 2007 a code of conduct for biosecurity has been proposed by the royal netherlands academy of. Responsibilities bso, ehs, ibc, pi, lab personnel training. Appendix c laboratory biosafety level 1 and 2 criteria.
Who laboratory manual for the examination and processing. Laboratory biosafety current staff university of queensland. Who laboratory manual for the examination and processing of. Laboratory biosecurity concepts are introduced, and the latest regulations for the transport of infectious substances are reflected.
Country and region overviews biosecurity challenges of. National biosafety guidelines evolved from the efforts of the microbiological and. The second edition of the cbs updates the biosafety standard originally published as part i of the canadian biosafety standards and guidelines cbsg, 1 st edition, 20. Cdcaphl laboratory biosafety competencies for the bsl2, bsl. Such specimens should be labeled accordingly, and the laboratory should be alerted to ensure proper specimen handling. Who laboratory biosafety manual updates absa conference. Cdc and the association of public health laboratories.
Cohen, centers for disease control and prevention, atlanta, ga, usa. Roles of biosafety laboratory personnel, by field and level biosafety level competency survey, united states, 2010. Sample laboratory biosafety manual for microbiological activities. Wcln resources wisconsin state laboratory of hygiene. Principal investigator, laboratory manager, postdoctoral student, or senior or staff scientist. Dec 07, 2010 as a followup to the november 30 biosafety presentation, a rudimentary, workinprogress version of the laboratory biosafety manual has been posted on the research integrity and compliance website.
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